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Molecular docking analysis of compounds 7c , 13a , and XV in LIMK1. Docking poses of (A) compound 7c (slate), (B) compound 13a (pink), and (C) compound XV (yellow) with LIMK1 (grey). The residues involved in the interaction are labelled as depicted. Hydrogen bonds are depicted as green dashed lines.

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Exploring diarylheptanoid derivatives to target LIMK1 as potential agents against colorectal cancer

doi: 10.1080/14756366.2025.2583826

Figure Lengend Snippet: Molecular docking analysis of compounds 7c , 13a , and XV in LIMK1. Docking poses of (A) compound 7c (slate), (B) compound 13a (pink), and (C) compound XV (yellow) with LIMK1 (grey). The residues involved in the interaction are labelled as depicted. Hydrogen bonds are depicted as green dashed lines.

Article Snippet: Furthermore, compound 13a complies with multiple medicinal chemistry filters, including Lipinski’s Rule of Five, Pfizer, GSK, and Golden Triangle criteria, collectively supporting its overall drug-likeness.

Techniques:

Inhibition activity of compound 13a against a panel of representative kinases.

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Exploring diarylheptanoid derivatives to target LIMK1 as potential agents against colorectal cancer

doi: 10.1080/14756366.2025.2583826

Figure Lengend Snippet: Inhibition activity of compound 13a against a panel of representative kinases.

Article Snippet: Furthermore, compound 13a complies with multiple medicinal chemistry filters, including Lipinski’s Rule of Five, Pfizer, GSK, and Golden Triangle criteria, collectively supporting its overall drug-likeness.

Techniques: Inhibition, Activity Assay

The anticancer effect of LIMK1 inhibitors. Cancer cells were treated with compound 13a at indicated concentrations (0.3, 1, 3, 10, and 30 μM) for 72 h in (A) HCT-116 and (B) HT-29 cells. The cell viability and cell proliferation were detected by MTT and SRB assays, respectively. All results present mean ± SD of three independent experiments. * p < 0.05 and ** p < 0.01 compared to control group (ctrl.).

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Exploring diarylheptanoid derivatives to target LIMK1 as potential agents against colorectal cancer

doi: 10.1080/14756366.2025.2583826

Figure Lengend Snippet: The anticancer effect of LIMK1 inhibitors. Cancer cells were treated with compound 13a at indicated concentrations (0.3, 1, 3, 10, and 30 μM) for 72 h in (A) HCT-116 and (B) HT-29 cells. The cell viability and cell proliferation were detected by MTT and SRB assays, respectively. All results present mean ± SD of three independent experiments. * p < 0.05 and ** p < 0.01 compared to control group (ctrl.).

Article Snippet: Furthermore, compound 13a complies with multiple medicinal chemistry filters, including Lipinski’s Rule of Five, Pfizer, GSK, and Golden Triangle criteria, collectively supporting its overall drug-likeness.

Techniques: Control

Compound 13a induced cancer cell apoptosis. (A) HCT-116 and (B) HT-29 cells were treated with compound 13a with 1, 3, 10, and 30 μM for 72 h. The quantitative analysis of cell cycle distribution and percentage of sub-G1 population were analysed by flow cytometry. ** p < 0.01 compared to control group (ctrl.).

Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

Article Title: Exploring diarylheptanoid derivatives to target LIMK1 as potential agents against colorectal cancer

doi: 10.1080/14756366.2025.2583826

Figure Lengend Snippet: Compound 13a induced cancer cell apoptosis. (A) HCT-116 and (B) HT-29 cells were treated with compound 13a with 1, 3, 10, and 30 μM for 72 h. The quantitative analysis of cell cycle distribution and percentage of sub-G1 population were analysed by flow cytometry. ** p < 0.01 compared to control group (ctrl.).

Article Snippet: Furthermore, compound 13a complies with multiple medicinal chemistry filters, including Lipinski’s Rule of Five, Pfizer, GSK, and Golden Triangle criteria, collectively supporting its overall drug-likeness.

Techniques: Flow Cytometry, Control